This synthesis report summarises the findings from 51 semi-structured interviews (Table 1) conducted by Technopolis as part of a landscape review of bioimaging commissioned by Wellcome. The interviews were either scoping interviews conducted at the beginning of the landscape review or stakeholder interviews conducted as part of the main data collection. The interviews explored the views of a range of stakeholders with a view to complement gaps in the data gathered by the desk research and survey, and to deepen our understanding of nascent technologies/methodologies and their added benefits, the barriers affecting progress in the field of bioimaging in both High-Income Countries (HICs) and Low/Middle-Income Countries (LMICs) and potential interventions to mitigate the barriers and/or to support breakthrough and game-changing work in the field.